Scientists develop human antibody panel against snake venom

Posted 02 October 2018

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·       Paper published in Nature Communications shows the potential of applying modern recombinant antibody technology to the blight of snake envenomation

·       Collaboration between IONTAS, Cambridge, Instituto Clodomiro Picado (Universidad de Costa Rica), and Technical University of Denmark to develop fully human IgG antibodies that neutralise black mamba snake venom in vivo


Cambridge, UK, San José, COSTA RICA and Kongens Lyngby, DENMARK, 02 October 2018: IONTAS Limited (IONTAS), a leader in the discovery and optimisation of fully human antibodies, today announced a collaborative paper published in Nature Communications[1] with the Technical University of Denmark, and the Instituto Clodomiro Picado of the University of Costa Rica describing the development of a panel of human antibodies that neutralise elements of black mamba snake toxin in an in vivo model.

Each year, around two million people fall victim to snakebite envenoming, which leads to more than 100,000 deaths and approximately 400,000 cases of severe sequalae, such as amputation. Particularly, impoverished victims living in snake-infested areas of the tropics are at risk, and many bites are left untreated due to the unavailability of safe and effective antivenoms. Snakebite envenoming has recently been introduced on the World Health Organization’s list of neglected tropical diseases due to its high disease burden.

The “proof of concept” research described in Nature Communications identified key components, including dendrotoxins, in the black mamba’s venom which contribute to venom toxicity. Human antibodies were generated to these dendrotoxins using IONTAS Phage Display Technology and cocktails of IgG-formatted human antibodies were then shown to protect mice from dendrotoxin-mediated neurotoxicity in vivo.

Associate Professor Andreas Hougaard Laustsen, Technical University of Denmark, commented: “Current antivenoms are based on plasma-derived animal antibodies, which are effective in neutralising venom toxicity, but are also associated with serious adverse reactions, such as serum sickness, due to their non-human origin. With our work we have laid the first stone on the technological path towards the manufacture of a next-generation fully human antivenom, devoid of such drawbacks.”

Professor José María Gutiérrez, Instituto Clodomiro Picado, Universidad de Costa Rica, said: “It is rewarding to see that classic techniques in toxinology and state-of-the-art methodologies of recombinant DNA could be successfully combined to demonstrate clear therapeutic potential. This recombinant antibody approach opens the door to the development of novel tools in the treatment of snakebites.”

Dr John McCafferty, Founder and CEO, IONTAS said: “Snake envenomation is a particular burden among the world’s poorest people and the approach to treatment, based on poorly defined animal serum has not changed in decades. IONTAS were motivated to contribute our resources and experience in recombinant antibody technology towards this initial proof-of-concept study. We hope that this report will help encourage funders to support the scientific community and advance the treatment of snakebites using modern antibody engineering methods. Although many challenges remain in the development of safe, efficacious and cost-effective drug cocktails, it is an achievable goal with the ultimate reward of seeing science help improve human lives.”

 

[1] https://www.nature.com/articles/s41467-018-06086-4

DOI 10.1038/s41467-018-06086-4

 

About IONTAS www.iontas.co.uk  

IONTAS is a biotechnology company focused on antibody discovery and cutting-edge technology development directed toward protein-based drug discovery. IONTAS was founded by John McCafferty principle inventor of antibody phage display technology and a co-founder of Cambridge Antibody Technology (now Medimmune, Cambridge). IONTAS offers services for antibody discovery using Phage Display Technology; and the supply of bespoke phage display libraries. In addition, IONTAS offers proprietary antibody discovery platforms including Mammalian Display where full length antibodies are expressed in the context of a mammalian cell thereby allowing selection based on function, stability, expression and developability; IONTAS have also developed a novel “KnotBody” format which facilitates the targeting of antibodies to ion channels, GPCRs and proteases. 

About Instituto Clodomiro Picado www.icp.ucr.ac.cr

 The Instituto Clodomiro Picado is a research center in Coronado, San José Province, Costa Rica. Established in 1970, the institute is a research unit of the Universidad de Costa Rica, responsible for the development and production of snake antivenoms and scientific research on snakes and their venoms, as well as educational and extension programs in rural areas and hospitals. The center collaborates internationally in research in Toxinology and Herpetology, working with more than 115 organizations in 31 different countries.

About Technical University of Denmark www.dtu.dk/english

DTU is recognized internationally as a leading university in the areas of technical and natural sciences, renowned for their business-oriented approach, focus on sustainability and study environment. The university was founded in 1829, and is now ranked as one of the foremost technical universities in Europe, continuing to set new records in the number of publications, and increase and develop partnerships with industry, and assignments accomplished by DTU’s scientific advice.