IONTAS will be attending and also presenting our latest poster titled "Mammalian display: antibody discovery and developability screening" PEGS Boston 2019, between 8th - 12th April.
Poster abstract: Using gene editing we have constructed large libraries of monoclonal stable cell lines where each cell contains a single antibody gene/cell inserted at a single locus. IgG-formatted antibodies are displayed on the cell surface permitting selection from the library of clones encoding desirable affinity, specificity, species cross-reactivity and "developability" properties using flow cytometry. Two case studies will be presented where antibodies with well documented poor biophysical characteristics were modelled to identify surface hydrophobic and positive charge patches, a library of variants created, and mammalian display used to select for antibodies with a superior developability profile. NGS analysis of selected antibody populations revealed sequence – developability relationships.
If you wish to meet to discuss mammalian display or your antibody discovery requirements please contact firstname.lastname@example.org