Mike Dyson PhD

Mike Dyson PhD

Chief Technology Officer

Biography

Mike has over 20 years experience in protein and small molecule chemistry, molecular biology and assay development gained during research at the Massachusetts Institute of Technology, Universities of Edinburgh and Cambridge and the biotechnology industry.

He has published 30 papers and book chapters (cited over 1300 times, h-index 19), edited a book on protein expression and is inventor on 5 US patent and patent applications. He was previously Head of Protein Engineering at Acambis plc, Group Leader at Sense Proteomic Ltd and was the Project Leader, responsible for protein expression, within the Atlas Project at the Wellcome Trust Sanger Institute.

He constructed the IONTAS antibody phage display library and acts as the project leader for several partnered therapeutic antibody discovery projects.

Publications by Mike

Fundamentals of Expression in Mammalian cells

Publication: Adv Exp Med Biol.. May 2016, 896 (217-24). PMID: 27165328.

Authors: Dyson MR

Selection of Antibodies Interfering with Cell Surface Receptor Signaling Using Embryonic Stem Cell Differentiation

Publication: Methods Mol. Biol. January 2016. 1341: 111-32 PMID: 26036698.

Authors: Melidoni AN, Dyson, MR, McCafferty J

Antibody-based exosite inhibitors of ADAMTS-5 (aggrecanase-2)

Publication: Biochem. J. November 2015, 471 (3): 391-401 PMID: 26303525.

Authors: Santamaria S, Yamamoto K, Botkjaer K,, Tape C, Dyson MR, McCafferty J, Murphy G, Nagase H

Expression screening in mammalian suspension cells

Publication: Methods Mol. Biol.. October 2013. 1091:143-9 doi:10.1007/978-1-62703-691-7_9 PMID: 24203329.

Authors: Chapple SD, Dyson MR

Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells

Publication: Proc. Natl. Acad. Sci. USA. September 2013. doi:10.1073/pnas.1312062110 PMID: 24143810.

A commentary summarising the significance of this paper has been published: Dalziel M, Crispin M, Dwek RA (2013) "Directing stem cell differentiation with antibodies." Proc. Natl. Acad. Sci. USA, Oct 29; 110(44): 17608-9. PMID: 24143810

Authors: Melidoni AN, Dyson MR, Wormald S, McCafferty J

Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells

Publication: Methods. September 2012, 58 (1): 69-78 PMID: 22842086.

Authors: Falk R, Falk A, Dyson MR, Melidoni AN, Parthiban K, Young JL, Roake W, McCafferty J

Detection of weak receptor-ligand interactions using IgM and J-chain-based fusion proteins

Publication: Eur. J. Immunol. May 2012, 42 (5): 1354-6 doi:10.1002/eji.201142151 PMID: 22539303.

Authors: Ammann JU, Jahnke M, Dyson MR, Kauffman J, Trowsdale J

Mapping protein interactions by combining antibody affinity maturation and mass spectroscopy

Publication: Anal Biochem.. May 2011, 417 (1): 25-35 doi:10.1016/j.ab.2011.05.005 PMID: 21704603.

Authors: Dyson MR, Zheng Y, Zhang C, Colwill K, Pershad K, Kay BK, Pawson T, McCafferty J

A roadmap to generate renewable protein binders to the human proteome.

Publication: Nat Methods. May 2011, 15 (8): 551-8 PMID: 21572409.

Authors: Collwil, K, Graslund, S, Renewable Protein Binder Working Group, Renewable Protein Binder Working Group, , , , , , , ,

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Selection of soluble protein expression constructs: the experimental determination of protein domain boundaries

Publication: Biochem. Soc. Trans.. August 2010, 38 (4): 908-13 doi:10.1042/BST0380908 PMID: 20658975.

Authors: Dyson MR

Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display

Publication: Protein Eng. des. Sel.. April 2010, 23 (4): 279-88 doi:10.1093/protein/gzq003 PMID: 20164219.

Authors: Pershad K, Pavlovic JD, Gräslund S, Nilsson P, Colwill K, Karatt-Vellatt A, Schofield DJ, Dyson MR, Pawson T, Kay BK, McCafferty J

Identification of soluble protein fragments by gene fragmentation and genetic selection

Publication: Nucleic Acids Res.. May 2008, 36 (9): e51 doi:10.1093/nar/gkn151 PMID: 18420658.

Authors: Dyson MR, Perera RL, Shadbolt SP, Biderman L, Bromek K, Murzina NV, McCafferty J

Application of phage display to high throughput antibody generation and characterisation

Publication: Genome Biol.. 2007, 8 (11): R254 doi:10.1186/gb-2007-8-11-r254 PMID: 18047641.

Authors: Schofield DJ, Pope AR, Clementel V, Buckell J, Chapple SD, Clarke KF, Conquer JS, Crofts AM, Crowther SR, Dyson MR, Flack G, Griffin GJ, Hooks Y, Howat WJ, Kolb-Kokocinski A, Kunze S, Martin CD, Maslen GL, Mitchell JN, O'Sullivan M, Perera RL, Roake W, Shadbolt SP, Vincent KJ, Warford A, Wilson WE, Xie J, Young JL, McCafferty J

Multiplexed expression and screening for recombinant protein production in mammalian cells

Publication: BMC Biotech.. December 2006. 6: 49 doi:10.1186/1472-6750-6-49 PMID: 17187663.

Authors: Chapple SD, Crofts AM, Shadbolt SP, McCafferty J, Dyson MR

Production of soluble mammalian proteins in E. coli: identification of protein features that correlate with successful expression

Publication: BMC Biotech.. December 2004. 4: 32 doi:10.1186/1472-6750-4-32 PMID: 15598350.

Authors: Dyson MR, Shadbolt SP, Vincent KJ, Perera RL, McCafferty J