Mike has over 20 years experience in protein and small molecule chemistry, molecular biology and assay development gained during research at the Massachusetts Institute of Technology, Universities of Edinburgh and Cambridge and the biotechnology industry.
He has published 30 papers and book chapters (cited over 1300 times, h-index 19), edited a book on protein expression and is inventor on 5 US patent and patent applications. He was previously Head of Protein Engineering at Acambis plc, Group Leader at Sense Proteomic Ltd and was the Project Leader, responsible for protein expression, within the Atlas Project at the Wellcome Trust Sanger Institute.
He constructed the IONTAS antibody phage display library and acts as the project leader for several partnered therapeutic antibody discovery projects.
Publications by Mike
A comprehensive search of functional sequence space using large mammalian display libraries created by gene editing
Fundamentals of Expression in Mammalian cells
Publication: Adv Exp Med Biol.. May 2016, 896 (217-24). PMID: 27165328.
Authors: Dyson MR
Selection of Antibodies Interfering with Cell Surface Receptor Signaling Using Embryonic Stem Cell Differentiation
Publication: Methods Mol. Biol. January 2016. 1341: 111-32 PMID: 26036698.
Authors: Melidoni AN, Dyson, MR, McCafferty J
Antibody-based exosite inhibitors of ADAMTS-5 (aggrecanase-2)
Publication: Biochem. J. November 2015, 471 (3): 391-401 PMID: 26303525.
Authors: Santamaria S, Yamamoto K, Botkjaer K,, Tape C, Dyson MR, McCafferty J, Murphy G, Nagase H
Expression screening in mammalian suspension cells
Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells
A commentary summarising the significance of this paper has been published: Dalziel M, Crispin M, Dwek RA (2013) "Directing stem cell differentiation with antibodies." Proc. Natl. Acad. Sci. USA, Oct 29; 110(44): 17608-9. PMID: 24143810
Authors: Melidoni AN, Dyson MR, Wormald S, McCafferty J
Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells
Publication: Methods. September 2012, 58 (1): 69-78 PMID: 22842086.
Authors: Falk R, Falk A, Dyson MR, Melidoni AN, Parthiban K, Young JL, Roake W, McCafferty J
Detection of weak receptor-ligand interactions using IgM and J-chain-based fusion proteins
Mapping protein interactions by combining antibody affinity maturation and mass spectroscopy
A roadmap to generate renewable protein binders to the human proteome.
Publication: Nat Methods. May 2011, 15 (8): 551-8 PMID: 21572409.
Authors: Collwil, K, Graslund, S, Renewable Protein Binder Working Group, Renewable Protein Binder Working Group, , , , , , , ,
Selection of soluble protein expression constructs: the experimental determination of protein domain boundaries
Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display
Identification of soluble protein fragments by gene fragmentation and genetic selection
Application of phage display to high throughput antibody generation and characterisation
Authors: Schofield DJ, Pope AR, Clementel V, Buckell J, Chapple SD, Clarke KF, Conquer JS, Crofts AM, Crowther SR, Dyson MR, Flack G, Griffin GJ, Hooks Y, Howat WJ, Kolb-Kokocinski A, Kunze S, Martin CD, Maslen GL, Mitchell JN, O'Sullivan M, Perera RL, Roake W, Shadbolt SP, Vincent KJ, Warford A, Wilson WE, Xie J, Young JL, McCafferty J