John McCafferty PhD

John McCafferty PhD

Founder / Chief Executive Officer


John was the pioneer and inventor of antibody-phage display and co-founder of Cambridge Antibody Technology which was subsequently acquired by MedImmune.

On leaving CAT, John established a highly proficient proteomics group at the Wellcome Trust Sanger Institute which developed cutting edge protein expression and high throughput recombinant antibody isolation.

More recently he headed a research group within the Department of Biochemistry, University of Cambridge focused on identifying anti-receptor antibodies with therapeutic potential in cancer indications.

Publications by John

A comprehensive search of functional sequence space using large mammalian display libraries created by gene editing

Publication: mAbs. June 2019. doi:10.1080/19420862.2019.1618673 PMID: 31107136.

Authors: Parthiban K, Perera RL, Sattar M, Huang Y, Masters E, Griffiths D, Surade S, Leah R, Dyson MR, McCafferty J

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In vivo neutralization of dendrotoxin-mediated neurotoxicity of black mamba venom by oligoclonal human IgG antibodies

Publication: Nature Communications. October 2018. doi:10.1038/s41467-018-06086-4 PMID: 30279409.

Authors: Laustsen AH, Karatt-Vellatt A, Masters EW, Arias AS, Pus U, Knudsen C, Oscoz S, Slavny P, Griffiths DT, Luther AM, Leah RA, Lindholm M, Lomonte B, Gutiérrez JM, McCafferty J

Basics of Antibody Phage Display Technology

Publication: Toxins (Basel). June 2018. doi:10.3390/toxins10060236 PMID: 29890762.

Authors: Ledsgaard L, Kilstrup M, Karatt-Vellatt A, McCafferty J, Laustsen AH

Characterization and structural determination of a new anti-MET function-blocking antibody with binding epitope distinct from ligand binding domain

Publication: Scientific Reports. August 2017. PMID: 28827556.

Authors: DiCara DM, Chirgadze DY, Pope AR, Karatt-Vellat A, Winter A, Slavny P, van den Heuvel J, Parthiban K, Holland J, Packman LC, Birchmeier W, Gherardi E, McCafferty J

Development of a specific affinity-matured exosite inhibitor to MT1-MMP that efficiently inhibits tumor cell invasion in vitro and metastasis in vivo

Publication: Oncotarget. March 2016, 29 (7): 16773-92 PMID: 26934448.

Authors: Botkjaer KA, Kwok HF, Terp MG, Karatt-Vellatt A, Santamaria S, McCafferty J, Andreasen PA, Itoh Y, Ditzel HJ, Murphy G

Selection of Antibodies Interfering with Cell Surface Receptor Signaling Using Embryonic Stem Cell Differentiation

Publication: Methods Mol. Biol. January 2016. 1341: 111-32 PMID: 26036698.

Authors: Melidoni AN, Dyson, MR, McCafferty J

The INNs and outs of antibody nonproprietary names.

Publication: MAbs. January 2016, 8 (1): 1-9 PMID: 26716992.

Authors: Jones TD, Carter PJ, Plückthun A, Vásquez M, Holgate RG, Hötzel I, Popplewell AG, Parren PW, Enzelberger M, Rademaker HJ, Clark MR, Lowe DC, Dahiyat BI, Smith V, Lambert JM, Wu H, Reilly M, Haurum JS, Dübel S, Huston JS, Schirrmann T, Janssen RA,, Steegmaier M, Gross JA, Bradbury AR, Burton DR, Dimitrov DS, Chester KA, Glennie MJ, Davies J, Walker A, Martin S, McCafferty J,, Baker MP

Antibody-based exosite inhibitors of ADAMTS-5 (aggrecanase-2)

Publication: Biochem. J. November 2015, 471 (3): 391-401 PMID: 26303525.

Authors: Santamaria S, Yamamoto K, Botkjaer K,, Tape C, Dyson MR, McCafferty J, Murphy G, Nagase H

Identification of optimal protein binders through the use of large genetically encoded display libraries

Publication: Curr . Opin. Chem. Biol.. June 2015. 26: 16-24 PMID: 25626152.

Authors: McCafferty J, Schofield D

Temporal- and embryonic lineage-dependent regulation of human vascular SMC development by Notch3

Publication: Stem Cells Dev.. December 2014. PMID: 25539150.

Authors: Granata A, Bernard WG, Zhao N, McCafferty J, Lilly B, Sinha S

Development of a 'mouse and human cross-reactive' affinity-matured exosite inhibitory human antibody specific to TACE (ADAM17) for cancer immunotherapy

Publication: Protein Eng. Des. Sel.. March 2014. 27:179 PMID: 24769623.

Authors: Kwok HF, Botkjaer KA, Tape CJ, McCafferty J, Murphy G

Phenotypic directed antibody selection

Publication: Chem. Biol.. February 2014, 21 (2): 170 doi:10.1016/j.chembiol.2014.02.004 PMID: 24560164.

Authors: McCafferty J

Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells

Publication: Proc. Natl. Acad. Sci. USA. September 2013. doi:10.1073/pnas.1312062110 PMID: 24143810.

A commentary summarising the significance of this paper has been published: Dalziel M, Crispin M, Dwek RA (2013) "Directing stem cell differentiation with antibodies." Proc. Natl. Acad. Sci. USA, Oct 29; 110(44): 17608-9. PMID: 24143810

Authors: Melidoni AN, Dyson MR, Wormald S, McCafferty J

Quantitative visualization of DNA G-quadruplex structures in human cells

Publication: Nature Chemistry. January 2013. doi:10.1038/nchem.1548

Authors: Biffi G, Tannahill D, McCafferty J, Balasubramanian S

Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells

Publication: Methods. September 2012, 58 (1): 69-78 PMID: 22842086.

Authors: Falk R, Falk A, Dyson MR, Melidoni AN, Parthiban K, Young JL, Roake W, McCafferty J

Recombinant antibodies and in vitro selection technologies

Publication: Methods Mol. Biol.. May 2012. 901:11-32 PMID: 22723092.

Authors: Geyer CR, McCafferty J, Dübel S, Bradbury AR, Sidhu SS

Mapping protein interactions by combining antibody affinity maturation and mass spectroscopy

Publication: Anal Biochem.. May 2011, 417 (1): 25-35 doi:10.1016/j.ab.2011.05.005 PMID: 21704603.

Authors: Dyson MR, Zheng Y, Zhang C, Colwill K, Pershad K, Kay BK, Pawson T, McCafferty J

A roadmap to generate renewable protein binders to the human proteome.

Publication: Nat Methods. May 2011, 15 (8): 551-8 PMID: 21572409.

Authors: Collwil, K, Graslund, S, Renewable Protein Binder Working Group, Renewable Protein Binder Working Group, , , , , , , ,

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Cross-domain inhibition of TACE ectodomain

Publication: Proc. Natl. Acad. Sci. USA. April 2011, 108 (14): 5578-83 doi:10.1042/BJ20100179 PMID: 21415364.

Authors: Tape CJ, Willems SH, Dombernowsky SL, Stanley PL, Fogarasi M, Ouwehand W, McCafferty J, Murphy G

Beyond natural antibodies: the power of in vitro display technologies

Publication: Nature Biotech.. March 2011, 29 (3): 245-54 doi:10.1038/nbt.1791 PMID: 21390033.

Authors: Bradbury AR, Sidhu S, Dübel S, McCafferty J

The long and winding road to antibody therapeutics

Publication: MAbs. Sep-Oct 2010, 2 (5): 459-60 doi:10.4161/mabs.2.5.13088 PMID: 20978369.

Authors: McCafferty J

Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display

Publication: Protein Eng. des. Sel.. April 2010, 23 (4): 279-88 doi:10.1093/protein/gzq003 PMID: 20164219.

Authors: Pershad K, Pavlovic JD, Gräslund S, Nilsson P, Colwill K, Karatt-Vellatt A, Schofield DJ, Dyson MR, Pawson T, Kay BK, McCafferty J

Control of feeding behavious in C. elegans by human G protein-coupled receptors permits screening for agonist-expressing bacteria

Publication: Proc. Natl. Acad. Sci. USA. September 2008, 105 (39): 14826-31 doi:10.1073/pnas.0803290105 PMID: 18815363.

Authors: Teng MS, Shadbolt P, Fraser AG, Jansen G, McCafferty J

Identification of soluble protein fragments by gene fragmentation and genetic selection

Publication: Nucleic Acids Res.. May 2008, 36 (9): e51 doi:10.1093/nar/gkn151 PMID: 18420658.

Authors: Dyson MR, Perera RL, Shadbolt SP, Biderman L, Bromek K, Murzina NV, McCafferty J

Application of phage display to high throughput antibody generation and characterisation

Publication: Genome Biol.. 2007, 8 (11): R254 doi:10.1186/gb-2007-8-11-r254 PMID: 18047641.

Authors: Schofield DJ, Pope AR, Clementel V, Buckell J, Chapple SD, Clarke KF, Conquer JS, Crofts AM, Crowther SR, Dyson MR, Flack G, Griffin GJ, Hooks Y, Howat WJ, Kolb-Kokocinski A, Kunze S, Martin CD, Maslen GL, Mitchell JN, O'Sullivan M, Perera RL, Roake W, Shadbolt SP, Vincent KJ, Warford A, Wilson WE, Xie J, Young JL, McCafferty J

Assessing the potential of immunohistochemistry for systematic gene expression profiling

Publication: J. Immunol. Methods. January 2007, 318 (1-2): 125-37 doi:10.1016/j.jim.2006.10.011 PMID: 17141799.

Authors: Warford A, Flack G, Conquer JS, Zola H, McCafferty J

ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome

Publication: Nature Meth.. January 2007, 4 (1): 13-7 doi:10.1038/nmeth0107-13 PMID: 17195019.

Authors: Taussig MJ, Stoevesandt O, Borrebaeck CA, Bradbury AR, Cahill D, Cambillau C, de Daruvar A, Dübel S, Eichler J, Frank R, Gibson TJ, Gloriam D, Gold L, Herberg FW, Hermjakob H, Hoheisel JD, Joos TO, Kallioniemi O, Koegl M, Konthur Z, Korn B, Kremmer E, Krobitsch S, Landegren U, van der Maarel S, McCafferty J, Muyldermans S, Nygren PA, Palcy S, Plückthun A, Polic B, Przybylski M, Saviranta P, Sawyer A, Sherman DJ, Skerra A, Templin M, Ueffing M, Uhlén M

Multiplexed expression and screening for recombinant protein production in mammalian cells

Publication: BMC Biotech.. December 2006. 6: 49 doi:10.1186/1472-6750-6-49 PMID: 17187663.

Authors: Chapple SD, Crofts AM, Shadbolt SP, McCafferty J, Dyson MR

A simple vector system to improve the performance and utilisation of recombinant antibodies

Publication: BMC Biotech. December 2006. 6: 49 doi:10.1186/1472-6750-6-46 PMID: 17156422.

Authors: Martin CD, Rojas G, Mitchell JN, Vincent KJ, Wu J, McCafferty J, Schofield DJ

Expression of mammalian GPCRs in C. elegans generates novel behavioural responses to human ligands

Publication: BMC Biol.. July 2006. 4: 22 PMID: 16857046.

Authors: Teng MS, Dekkers MP, Ng BL, Rademakers S, Jansen G, Fraser AG, McCafferty J

Resin tissue microarrays: a universal format for immunohistochemistry

Publication: J. Histochem. Cytochem.. October 2005, 53 (10): 1189-97 doi:10.1369/jhc.5C6659.2005 PMID: 15983117.

Authors: Howat WJ, Warford A, Mitchell JN, Clarke KF, Conquer JS, McCafferty J

Production of soluble mammalian proteins in E. coli: identification of protein features that correlate with successful expression

Publication: BMC Biotech.. December 2004. 4: 32 doi:10.1186/1472-6750-4-32 PMID: 15598350.

Authors: Dyson MR, Shadbolt SP, Vincent KJ, Perera RL, McCafferty J

Expression profiling by high throughput immunohistochemistry

Publication: J. Immunol. Methods. July 2004, 290 (102): 81-92 doi:10.1016/j.jim.2004.04.010 PMID: 15261573.

Authors: Warford A, Howat W, McCafferty J