John was the pioneer and inventor of antibody-phage display and co-founder of Cambridge Antibody Technology which was subsequently acquired by MedImmune.
On leaving CAT, John established a highly proficient proteomics group at the Wellcome Trust Sanger Institute which developed cutting edge protein expression and high throughput recombinant antibody isolation.
More recently he headed a research group within the Department of Biochemistry, University of Cambridge focused on identifying anti-receptor antibodies with therapeutic potential in cancer indications.
Publications by John
A comprehensive search of functional sequence space using large mammalian display libraries created by gene editing
In vivo neutralization of dendrotoxin-mediated neurotoxicity of black mamba venom by oligoclonal human IgG antibodies
Basics of Antibody Phage Display Technology
Characterization and structural determination of a new anti-MET function-blocking antibody with binding epitope distinct from ligand binding domain
Publication: Scientific Reports. August 2017. PMID: 28827556.
Authors: DiCara DM, Chirgadze DY, Pope AR, Karatt-Vellat A, Winter A, Slavny P, van den Heuvel J, Parthiban K, Holland J, Packman LC, Birchmeier W, Gherardi E, McCafferty J
Development of a specific affinity-matured exosite inhibitor to MT1-MMP that efficiently inhibits tumor cell invasion in vitro and metastasis in vivo
Publication: Oncotarget. March 2016, 29 (7): 16773-92 PMID: 26934448.
Authors: Botkjaer KA, Kwok HF, Terp MG, Karatt-Vellatt A, Santamaria S, McCafferty J, Andreasen PA, Itoh Y, Ditzel HJ, Murphy G
Selection of Antibodies Interfering with Cell Surface Receptor Signaling Using Embryonic Stem Cell Differentiation
Publication: Methods Mol. Biol. January 2016. 1341: 111-32 PMID: 26036698.
Authors: Melidoni AN, Dyson, MR, McCafferty J
The INNs and outs of antibody nonproprietary names.
Publication: MAbs. January 2016, 8 (1): 1-9 PMID: 26716992.
Authors: Jones TD, Carter PJ, Plückthun A, Vásquez M, Holgate RG, Hötzel I, Popplewell AG, Parren PW, Enzelberger M, Rademaker HJ, Clark MR, Lowe DC, Dahiyat BI, Smith V, Lambert JM, Wu H, Reilly M, Haurum JS, Dübel S, Huston JS, Schirrmann T, Janssen RA,, Steegmaier M, Gross JA, Bradbury AR, Burton DR, Dimitrov DS, Chester KA, Glennie MJ, Davies J, Walker A, Martin S, McCafferty J,, Baker MP
Antibody-based exosite inhibitors of ADAMTS-5 (aggrecanase-2)
Publication: Biochem. J. November 2015, 471 (3): 391-401 PMID: 26303525.
Authors: Santamaria S, Yamamoto K, Botkjaer K,, Tape C, Dyson MR, McCafferty J, Murphy G, Nagase H
Identification of optimal protein binders through the use of large genetically encoded display libraries
Publication: Curr . Opin. Chem. Biol.. June 2015. 26: 16-24 PMID: 25626152.
Authors: McCafferty J, Schofield D
Temporal- and embryonic lineage-dependent regulation of human vascular SMC development by Notch3
Publication: Stem Cells Dev.. December 2014. PMID: 25539150.
Authors: Granata A, Bernard WG, Zhao N, McCafferty J, Lilly B, Sinha S
Development of a 'mouse and human cross-reactive' affinity-matured exosite inhibitory human antibody specific to TACE (ADAM17) for cancer immunotherapy
Publication: Protein Eng. Des. Sel.. March 2014. 27:179 PMID: 24769623.
Authors: Kwok HF, Botkjaer KA, Tape CJ, McCafferty J, Murphy G
Phenotypic directed antibody selection
Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells
A commentary summarising the significance of this paper has been published: Dalziel M, Crispin M, Dwek RA (2013) "Directing stem cell differentiation with antibodies." Proc. Natl. Acad. Sci. USA, Oct 29; 110(44): 17608-9. PMID: 24143810
Authors: Melidoni AN, Dyson MR, Wormald S, McCafferty J
Quantitative visualization of DNA G-quadruplex structures in human cells
Publication: Nature Chemistry. January 2013. doi:10.1038/nchem.1548
Authors: Biffi G, Tannahill D, McCafferty J, Balasubramanian S
Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells
Publication: Methods. September 2012, 58 (1): 69-78 PMID: 22842086.
Authors: Falk R, Falk A, Dyson MR, Melidoni AN, Parthiban K, Young JL, Roake W, McCafferty J
Recombinant antibodies and in vitro selection technologies
Publication: Methods Mol. Biol.. May 2012. 901:11-32 PMID: 22723092.
Authors: Geyer CR, McCafferty J, Dübel S, Bradbury AR, Sidhu SS
Mapping protein interactions by combining antibody affinity maturation and mass spectroscopy
A roadmap to generate renewable protein binders to the human proteome.
Publication: Nat Methods. May 2011, 15 (8): 551-8 PMID: 21572409.
Authors: Collwil, K, Graslund, S, Renewable Protein Binder Working Group, Renewable Protein Binder Working Group, , , , , , , ,
Cross-domain inhibition of TACE ectodomain
Beyond natural antibodies: the power of in vitro display technologies
The long and winding road to antibody therapeutics
Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display
Control of feeding behavious in C. elegans by human G protein-coupled receptors permits screening for agonist-expressing bacteria
Identification of soluble protein fragments by gene fragmentation and genetic selection
Application of phage display to high throughput antibody generation and characterisation
Authors: Schofield DJ, Pope AR, Clementel V, Buckell J, Chapple SD, Clarke KF, Conquer JS, Crofts AM, Crowther SR, Dyson MR, Flack G, Griffin GJ, Hooks Y, Howat WJ, Kolb-Kokocinski A, Kunze S, Martin CD, Maslen GL, Mitchell JN, O'Sullivan M, Perera RL, Roake W, Shadbolt SP, Vincent KJ, Warford A, Wilson WE, Xie J, Young JL, McCafferty J
Assessing the potential of immunohistochemistry for systematic gene expression profiling
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome
Authors: Taussig MJ, Stoevesandt O, Borrebaeck CA, Bradbury AR, Cahill D, Cambillau C, de Daruvar A, Dübel S, Eichler J, Frank R, Gibson TJ, Gloriam D, Gold L, Herberg FW, Hermjakob H, Hoheisel JD, Joos TO, Kallioniemi O, Koegl M, Konthur Z, Korn B, Kremmer E, Krobitsch S, Landegren U, van der Maarel S, McCafferty J, Muyldermans S, Nygren PA, Palcy S, Plückthun A, Polic B, Przybylski M, Saviranta P, Sawyer A, Sherman DJ, Skerra A, Templin M, Ueffing M, Uhlén M
Multiplexed expression and screening for recombinant protein production in mammalian cells
A simple vector system to improve the performance and utilisation of recombinant antibodies
Expression of mammalian GPCRs in C. elegans generates novel behavioural responses to human ligands
Publication: BMC Biol.. July 2006. 4: 22 PMID: 16857046.
Authors: Teng MS, Dekkers MP, Ng BL, Rademakers S, Jansen G, Fraser AG, McCafferty J